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streptrap xt  (Cytiva Europe)


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    Structured Review

    Cytiva Europe streptrap xt
    Streptrap Xt, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/streptrap+xt/StrepTrap+XT/custom%4029401317%4042424095
    Average 95 stars, based on 221 article reviews
    streptrap xt - by Bioz Stars, 2026-09
    95/100 stars

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    Purification:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Concentration Assay:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Centrifugation:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Lysis:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Sonication:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Recombinant:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Chromatography:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Affinity Column:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Incubation:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Virus:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Expressing:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Expression Vector:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Clone Assay:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Plasmid Preparation:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Affinity Purification:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Synthesized:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    SDS Page:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F

    Affinity Chromatography:

    Article Title: Inorganic phosphate rapidly switches the stability of Arp2/3-induced actin branches
    Article Snippet: ysate was then clarified by ultracentrifugation at 190,000 g for 45 min at 4°C, followed by passage through a 0.45-μm syringe filter. The resulting filtrate was loaded onto a 1 ml StrepTrap XT column (Cytiva). After washing with the purification buffer, the bound Arp2/3 complex was eluted with buffer BXT (IBA) supplemented with 2 mM ATP, 1 mM DTT, 5 mM EGTA, 2 mM MgCl2, and 2.5% vol/vol glycerol. F



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